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L-isoleucine biosynthesis I (from threonine)

State Icon Reaction EC-Number Confidence maximal confidence score from EnzymeDetector
H+ + pyruvate + 2-oxobutyrate <=> (S)-2-hydroxy-2-ethyl-3-oxobutanoate + CO2 2.2.1.6 acetolactate synthase

2R,3R-2,3-dihydroxy-3-methylpentanoate <=> (S)-3-methyl-2-oxopentanoate + H2O 4.2.1.9 dihydroxy-acid dehydratase

L-isoleucine + 2-oxoglutarate <=> L-glutamate + (S)-3-methyl-2-oxopentanoate 2.6.1.42 branched-chain-amino-acid transaminase
2.6.1.67 2-aminohexanoate transaminase


2-aminobut-2-enoate <=> 2-iminobutanoate 3.5.99.7 1-aminocyclopropane-1-carboxylate deaminase
4.3.1.19 threonine ammonia-lyase
4.4.1.1 cystathionine gamma-lyase
4.4.1.2 homocysteine desulfhydrase




2-iminobutanoate + H2O + H+ <=> 2-oxobutyrate + NH3 3.5.99.10 2-iminobutanoate/2-iminopropanoate deaminase
3.5.99.7 1-aminocyclopropane-1-carboxylate deaminase
4.3.1.19 threonine ammonia-lyase
4.4.1.1 cystathionine gamma-lyase
4.4.1.2 homocysteine desulfhydrase





L-threonine <=> 2-aminobut-2-enoate + H2O + H+ 4.3.1.19 threonine ammonia-lyase

2R,3R-2,3-dihydroxy-3-methylpentanoate + NADP+ <=> H+ + (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH 1.1.1.86 ketol-acid reductoisomerase (NADP+)

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